纳升微量显微操作泵300704

仪器概况

      WPI公司全新上市的纳升微量显微操作泵300704是在原NL2010经典产品的基础上升级换代而来。该产品除了具有NL 2000和NL 2010的操作方便、注射量精确外，还具备以下特征：
 1.操作泵整机采用铝合金材质生产，外观优美；
 2.操作泵直接连接控制器，无需转换适配器；
3. 操作泵采用专用玻璃毛细管确保注射体积准确；
4.操作泵尾部带有LED指示灯，用户可以通过它了解设备的运行状态；
      新版操作泵控制器中的软件进行了升级，可以设置操作泵的移动终点，防止泵头在操作过程中的过度移动导致马达的损坏。
      控制器上超大的LED显示屏可以显示两个通道的全部信息，包括但不限于显示正在使用的通道、工作状态（注射或回吸）、已注射体积及所占百分比、注射速度等信息。

仪器特征

注射体积范围大，从纳升到微升级。
注射精度高，体积增量为0.1纳升，注射重复性好；
仪器体积小巧，重量仅1.1公斤，不占实验室空间；
全亮银色铝合金机壳设计，时尚美观，耐磨耐腐蚀，经久耐用；
即可配合升级的双通道Micro2T控制器使用，也可连接经典的Micro4控制器使用；
兼容性强，即可固定在脑立体定位仪上对颅脑进行注射，也可固定在手动或电动显微操作臂上对昆
虫卵、斑马鱼或水产类卵细胞进行注射；

仪器应用

用于水产类研究：
用于水产类卵细胞DNA导入；
用于斑马鱼卵细胞遗传物质导入；
用于斑马鱼幼鱼RNA干扰和染料注射；
用于昆虫研究：
用于褐飞虱、稻飞虱和棉铃虫卵细胞注射；
用于果蝇卵细胞和幼蝇体内注射；
用于线虫、蠕虫和蚜虫体内注射；
用于啮齿动物研究：
用于大鼠和小鼠颅内药物和染料注射；
用于大鼠及小鼠胚胎RNA干扰注射；

参考文献

Vagal sensory neurons mediate the Bezold–Jarisch reflex and induce syncope
Nature 2023, 623:387–396
https://doi.org/10.1038/s41586-023-06680-7
Neomycin-induced deafness in neonatal mice
Journal of Neuroscience Methods 2023, 391:109852
https://doi.org/10.1016/j.jneumeth.2023.109852
Neuronal activity dynamics in the dentate gyrus during early epileptogenesis
Epilepsy Research 2023, 194:107182
https://doi.org/10.1016/j.eplepsyres.2023.107182
Neural and central mechanisms of kidney fibrosis after relief of ureteral obstruction 
iScience 2023, 26:106338
https://doi.org/10.1016/j.isci.2023.106338
Isolation and short-term culturing of primary lymphatic endothelial cells from collecting lymphatics: Atechniques study
Microcirculation. 2023, 30:e12778.
https://doi.org/10.1111/micc.12778
Nano-injection method for micro-insects without sedation using the western flower thrips, Frankliniella occidentalis
Journal of Applied Entomology 2022, 146(9):1200-1206
https://doi.org/10.1111/jen.13063
Amachine vision guided robot for fully automated embryonic microinjection
BioRxiv Posted April 2023
https://doi.org/10.1101/2023.04.25.538280
Probing Intracellular Potassium Dynamics in Neurons with the Genetically Encoded Sensor lc-LysM GEPII 1.0 in vitro and
in vivo
https://doi.org/10.21203/rs.3.rs-3715395/v1
Carbon nanospikes have improved sensitivity and antifouling properties for adenosine, hydrogen peroxide, and histamine
Anal Bioanal Chem 2023, 415:6039–6050
https://doi.org/10.1007/s00216-023-04875-5
Functional contribution of glutamatergic neurons of medullary reticular nuclei to locomotor recovery after spinal cord injury
https://doi.org/10.21203/rs.3.rs-3140362/v1
Increased miR-200c levels disrupt palatal fusion by affecting apoptosis, cell proliferation, and cell migration
Biochemical and Biophysical Research Communications 2023, 664:43-49
https://doi.org/10.1016/j.bbrc.2023.04.090
Anovel GSTe2 involved in metamorphosis by regulating 20E signal pathway in Tribolium castaneum
Insect Biochemistry and Physiology 2023, 112(3):e21989
https://doi.org/10.1002/arch.21989
Slow-rising and fast-falling dopaminergic dynamics jointly adjust negative prediction error in the ventral striatum
European Journal of Neuroscience 2023, 58(12):4502-4522
https://doi.org/10.1111/ejn.15945
Transformations of neural representations in a social behaviour network. 
Nature 2022, 608:741–749
https://doi.org/10.1038/s41586-022-05057-6
The role of somatosensory innervation of adipose tissues
Nature 2022, 609:569–574
https://doi.org/10.1038/s41586-022-05137-7