程控水平拉制仪
仪器概况
PUL-1000是一款微处理器控制的四步程序水平拉制仪,可以拉制用于细胞内信号记录、膜片钳研究、微量灌注及显微注射的玻璃毛细管或微电极。该拉制仪具有紧凑的结构和精密的电子设计,可完全控制拉制过程,并且具有准确的重复性。它可以编程包括加热、拉力、拉伸长度和延迟时间等四个参数。
安装于拉制仪上的玻璃毛细管一般由铂铱金属丝加热,然后通过微处理器控制的拉力、拉伸长度及延迟时间等完成拉制,PUL1000最多可以储存95个程序。通过这些储存的程序可以拉制不同大小的玻璃毛细管尖端。玻璃毛细管的拉制是一门技术活,拉制完好的玻璃毛细管尖端受多种因素的影响,如操作环境、不同厂家的玻璃毛细管、不同种类的玻璃毛细管、不同批号的玻璃毛细管以及操作人员的娴熟程度。
产品特征
仪器小巧,重量仅7公斤,不占空间便于移动;
钢化玻璃盖可减少环境温度和湿度对尖端拉制的影响;
拉制的尖端可重复性不受100-240伏特电压波动的影响;
可拉制的玻璃毛细管尖端最小0.1微米,最大可大于10微米;
可编程一步拉制注射用玻璃毛细管,多步拉制膜片钳专用电极;
从1.0到1.5毫米玻璃毛细管,出厂时都已设置一步和多步程序供参考;
内存可存储多达95个程序,供多人或多种不同规格的毛细管参数存储;
操作简便,在设置温度、拉力、长度和延时后,按压启动键即可;
参考文献
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Voltage and concentration gradients across membraneless interface generated next to hydrogels: relation to glycocalyx
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Nanocapillary sampling coupled to liquid chromatography mass spectrometry delivers single cell drug measurement
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Sterol derivative binding to the orthosteric site causes conformational changes in an invertebrate Cys-loop receptor
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Optogenetic Signaling Activation in Zebrafish Embryos
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Loss of Polycystin-1 causes cAMP-dependent switch from tubule to cyst formation
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Mimicry of a biophysical pathway leads to diverse pollen-like surface patterns
PNAS 2020, 117(18):9699-9705
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Pathogenicity of West Nile Virus Lineage 1 to German Poultry
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The Kinase Activity of Drosophila BubR1 Is Required for Insulin Signaling-Dependent Stem Cell Maintenance
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https://doi.org/10.1016/j.celrep.2020.107794