点针细胞电穿孔仪
产品简介
WPI公司最新上市的点针细胞电穿孔仪是一款简单、通用的细胞膜或细胞壁的穿透设备， 可用于将DNA、iRNA等遗传信息物质注入胚胎细胞以及植入前期的哺乳动物胚胎中，可在不撕裂或破坏细胞膜的情况下，实现小创伤、不留痕迹 、精确的膜穿透。
产品特征
● 触摸屏显示：电阻式触摸屏，戴手套也可方便使用; 仪器操作模式：脚踏开关控制注射或触摸屏手动注射控制 ;
● 直观的用户界面;占用空间小;
● 参数设置：通过触摸屏或旋钮调节频率和电压; 电穿孔程序：四种用户可编程程序;
● 警示音：可调音频连续音表示有效探头; 计数显示：注射计数器指示注射总次数;
● 通用性：电极支架与大多数常见操纵器兼容; 高存活率：小鼠胚胎双细胞期注射后约80-85% 存活率;
装在倒置显微镜下的点针细胞电穿孔仪。可以兼容WPI公司SYS-PV830，艾本德公司的FemtoJet注射系统。
这是一个典型的微注射装置，使用MICRO-ePORE™、一个INV-101倒置显微镜和一个PV830气动皮升操作泵。
产品用途
● 微量注射到卵母细胞和植入前期的哺乳动物胚胎, 包括将CRISPR-Cas9试剂注射到双细胞胚胎的细胞质中;
● 原核啮齿动物受精卵微量注射;
● 斑马鱼中的基因沉默;
江西某高校斑马鱼中心使用第二代原型机在斑马鱼尾鳍注入Morphilinos。
参考文献
[1] Allocation of cells to inner cell mass and trophecto
-derm lineages in preimplantation mouse embryos. Dev Biol. 1982, 90(2):352-62. PMID: 7075865
https://www.ncbi.nlm.nih.gov/pubmed/7075865
[2] Cell fate, morphogenetic movement and population kinetics of embryonic endoderm at the time of germ layer formation in the mouse.
Development. 1987, 101(3):627-52. PMID:3502998
https://www-ncbi-nlm-nih.gov.myaccess.library. utoronto.ca/pubmed/3502998
[3] Specific interference with gene function by double
-stranded RNA in early mouse development.
Nat Cell Biol. 2000, 2(2):70-5. PMID:10655585
https://www.ncbi.nlm.nih.gov/pubmed/10655585
[4] Early lineage segregation between epiblast and pri
-mitive endoderm in mouse blastocysts through the Grb2-MAPK pathway.
Dev Cell. 2006 May; 10(5):615- 24. PMID:16678776
https://www.ncbi.nlm.nih.gov/pubmed/16678776
[5] Use of luciferase chimaera to monitor PLCzeta exp
-ression in mouse eggs. Methods Mol Biol. 2009; 518:17-29. PMID:19085135
https://www.ncbi.nlm.nih.gov/pubmed/19085135
[6] Position- and Hippo signaling-dependent plasticity during lineage segregation in the early mouse embryo. Elife. 2017 Feb 22; 6. pii: e22906. PMID: 28226240
https://www.ncbi.nlm.nih.gov/pubmed/28226240
[7] Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Nature Biotechnology 2018, 36(7):632-637. PMID: 29889212 https://www.ncbi.nlm.nih.gov/pubmed/29889212
[8] Glycolysis-Independent Glucose Metabolism Disti
-nguishes TE from ICM Fate during Mammalian Embryogenesis.
Developmental Cell 2020, 53, 9–26 https://doi.org/10.1016/j.devcel.2020.02.015
