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气体信号分子和生物自由基检测仪TBR4100
产品简介
一氧化氮(NO)、一氧化碳(CO)和硫化氢(H2S)已经在动物和植物体内证实为气体信号分子,并在动物和植物 生理和病理过程中发挥重要的信使作用。生物自由基除了包括上述分子外,还有过氧化氢(HPO)、羟基和超氧阴离子等也在动物和植物的生理和病理过程中发挥重要的作用。因此,对气体信号分子和生物自由基的检测就成为动物和植物生理研究的重要方法。
国产/进口:进口
产地/品牌:WPI
型        号:TBR4100
货        号:TBR4100
  • 产品详情
  • 产品参数
  • 产品用途
产品概况
一氧化氮(NO)、一氧化碳(CO)和硫化氢(H2S)已经在动物和植物体内证实为气体信号分子,并在动物和植物 生理和病理过程中发挥重要的信使作用。生物自由基除了包括上述分子外,还有过氧化氢(HPO)、羟基和超氧阴离子等也在动物和植物的生理和病理过程中发挥重要的作用。因此,对气体信号分子和生物自由基的检测就成为动物和植物生理研究的重要方法。
由于NO、H2S和HPO专利的100微米组织电极的成功开发,因此该设备是市场上唯一的可对动物或植物体内上述分子进行实时在体检测的设备。
产品特征
◆ 检测范围大:
NO的检测范围:0.3 nM-100 μM H 2 S的检测范围:5 nM-100 μM CO的检测范围:10 nM-10 μM
HPO的检测范围:10 nM-100 mM Glucose的检测范围:2-25 mM/L O 2 的检测范围:0.1-100 %
◆ 灵敏度高:
NO的检测灵敏度:1-10 pA/nM H 2 S的检测灵敏度:0.5-2 pA/nM
CO的检测灵敏度:0.5 pA/nM HPO的检测灵敏度:0.02-4 pA/nM O 2 的检测灵敏度:0.3-0.6 nA/%
◆ 空间分辨率高:H 2 S、NO 和 HPO 均具有100 μm及以下电极,用于组织内分子检测时空间分辨率极高
◆ 快速反应时间:NO:<5 s ; H 2 S:<5 s ; HPO:<5 s ; O 2 :<10 s ; CO:<10 s
◆ 一机多用,可以同时检测NO、H 2 S、HPO、CO、Glucose 和 O 2
◆ 操作简单,标定方便,用户友好的软件操作系统
◆ 具有单通道和四通道可选
◆不用类型(L型、H型和Z型)电极可选,可以应用于不同的研究
参考文献
用于植物应激等研究参考文献
[1] Salinity-induced accumulation of endogenous H 2 S and NO is associated with modulation of the antioxidant
and redox defense systems in Nicotiana tabacum L. cv. Havana
Plant Science 256 (2017) 148–159
[2] Hydrogen sulfide mediates ion fluxes inducing stomatal closure in response to drought stress in Arabidopsis
thaliana
Plant and Soil 2017, 419(1-2):141–152;
https://doi.org/10.1007/s11104-017-3335-5
[3] Characterization of the Heme Pocket Structure and Ligand Binding Kinetics of Non-symbiotic Hemoglobins
from the Model Legume Lotus japonicus
Front Plant Sci. 2017; 8: 407.
https://doi.org/10.3389/fpls.2017.00407
[4] The Ca2+/calmodulin2-binding transcription factor TGA3 elevates LCD expression and H2S production to
bolster Cr6+ tolerance in Arabidopsis
The Plant Journal (2017):1-13
https://doi.org/10.1111/tpj.13627
[5] Exogenous nitric oxide improves sugarcane growth and photosynthesis under water deficit[J].
Planta, 2016, 244, 181-190.
[6] Characterization of iron nanoparticles produced with green tea extract: a promising material for nitric oxide
delivery.
Biointerface Research in Applied Chemistry . 2016, 6(3):1280-1287;
[7] Enhanced chloroplastic generation of H2O2in stress-resistant Thellungiella salsuginea in comparison to
Arabidopsis thaliana[J].
Physiologia plantarum, 2015, 153.3, 467-476.
[8] Impairment in Sulfite Reductase Leads to Early Leaf Senescence in Tomato Plants
Plant Physiology,2014,165:1505–1520;
[9] Calcium-sensing receptor regulates stomatal closure through hydrogen peroxide and nitric oxide in response
to extracellular calcium in Arabidopsis[J].
Journal of Experimental Botany, 2012, 63(1), 177-190.
[10] Specificity of Polyamine Effects on NaCl-induced Ion Flux Kinetics and Salt Stress Amelioration in
Plants[J].
Plant Cell Physiol., 2010, 51(3), 422-434.
用于缺血再灌注和糖尿病、肥胖研究的参考文献
[1]Apocynin Exerts Dose-Dependent Cardioprotective Effects by Attenuating Reactive Oxygen Species in Ischemia/Reperfusion[J].
CardiovascPharm Open Access, 2016, 5(2), 1000176.
[2] Murine strain differences in inflammatory angiogenesis of internal wound in diabetes.
Biomedicine & Pharmacotherapy, 2017,86:715-724
https://doi.org/10.1016/j.biopha.2016.11.146
[3] Delivery of Hydrogen Sulfde by Ultrasound Targeted Microbubble Destruction Attenuates Myocardial
Ischemia-reperfusion Injury[J].
Scientific Reports, 2016, 6, 30643-30656.
[4] Effects of Mitochondrial-Targeted Antioxidants on Real-Time Blood Nitric Oxide and Hydrogen Peroxide
Release in Acute Hyperglycemic Rats[J].
Proceedings of the 24th American Peptide Symposium, American Peptide Society, 2015.
[5] Lipopolysaccharide-induced cross-tolerance against renal ischemia–reperfusion injury is mediated by
hypoxia-inducible factor-2a-regulated nitric oxide production[J].
Kidney International, 2014, 85, 276-288.
[6] Gp91ds-tat, a selective NADPH oxidase peptide inhibitor, increases blood nitric oxide bioavailability in hind
limb ischemia and reperfusion[J].
Faseb Journal, 2014, 28.
[7] Lipocalin-2 deficiency prevents endothelial dysfunction associated with dietary obesity: role of cytochrome
P450 2C inhibition.
British Journal of Pharmacology,2012,165(2):520–531
[8] Abstract 3929: Basis of Impaired Coronary Metabolic Dilation in Obesity and Insulin Resistance.
Circulation,2008, 118(S18)
[9] Tumor Necrosis Factor-α Induces Endothelial Dysfunction in the Prediabetic Metabolic Syndrome
Circulation Research,2006; 99: 69-77
[10]Hypercontractile Female Hearts Exhibit Increased S-Nitrosylation of the L-Type Ca2+ Channel α1 Subunit
and Reduced Ischemia/Reperfusion Injury
Circulation Research,2006; 98: 403-411
用于血管内皮及高血压研究参考文献
[1] N-Salicyloyltryptamine, an N-Benzoyltryptamine Analogue, Induces Vasorelaxation through Activation
of the NO/sGC Pathway and Reduction of Calcium Influx.
Molecules 2018, 23, 253;
https://doi.org/10.3390/molecules23020253
[2] NO production and potassium channels activation induced by Crotalus durissus cascavella underlie
mesenteric artery relaxation.
Toxicon. 2017, 133:10-17
[3] H2S inhibits pulmonary arterial endothelial cell inflammation in rats with monocrotaline-induced pulmonary
hypertension.
Laboratory Investigation, 2017,97:268–278;
https://doi.org/10.1038/labinvest.2016.129
[4] Hydrogen-sulfide-mediated vasodilatory effect of nucleoside 5′-monophosphorothioates in perivascular
adipose tissue.
Canadian Journal of Physiology and Pharmacology, 2015, 93(7): 585-595(引用1次)
[5] Vasorelaxation Induced by a New Naphthoquinone-Oxime is Mediated by NO-sGC-cGMP Pathway.
Molecules 2014, 19(7):9773-9785
[6] Hydrogen sulfide upregulates KATP channel expression in vascular smooth muscle cells of spontaneously
hypertensive rats.
Journal of Molecular Medicine,2015,93(4):439-455
[7] Down-regulated CBS/H2S pathway is involved in high-salt-induced hypertension in Dahl rats.
Nitric Oxide,2015, 46:192–203
[8] Elevated Inducible Nitric Oxide Levels and Decreased Hydrogen Sulfide Levels Can Predict the Risk of Coronary Artery Ectasia in Kawasaki Disease.
Pediatric Cardiology,2016, 37(2):322–329
[9] Mechanism of the Vasodilator Effect of Mono-oxygenated Xanthones: A Structure-Activity Relationship Study.
Planta Med 2013; 79(16): 1495-1500
[10] Angiopreventive versus angiopromoting effects of allopurinol in the murine sponge model.
Microvascular Research,2015,101:118–126
[11] Hydrogen Peroxide Generated From Cardiac Myocytes Impacts Metabolic Dilation in Coronary Arterioles.
International Heart Journal,2010,51(2):125-128
[12] l-Theanine promotes nitric oxide production in endothelial cells through eNOS phosphorylation
The Journal of Nutritional Biochemistry,2013,24(3):595–605
[13] Shear stress promotes nitric oxide production in endothelial cells by sub-cellular delocalization of eNOS:
A basis for shear stress mediated angiogenesis.
Nitric Oxide,2010,22(4):304–315
用于纳米材料研究参考文献
[1] Conjugating S-nitrosothiols with fluorescent nanofibers for the controlled release and real-time detection of
nitric oxide.
Fibers and Polymers,2016, 17(6):971–975
[2] Co-delivery of nitric oxide and antibiotic using polymeric nanoparticles.
Chem. Sci., 2016, 7, 1016
https://DOI.org/10.1039/c5sc02769a
[3] Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical
applications.
RSC Adv., 2017, 7, 14496–14503
https://DOI.org/10.1039/c6ra28633j
[4] A multifunctional nanoplatform for lysosome targeted delivery of nitric oxide and photothermal therapy under
808 nm near-infrared light.
Journal of Materials Chemistry B, 2016, 27: 4 : 4667-4674
https://DOI.org/10.1039/C6TB00730A
[5] Ruthenium nitrosyl grafted carbon dots as a fluorescence-trackable nanoplatform for visible light-controlled
nitric oxide release and targeted intracellular delivery.
Journal of Inorganic Biochemistry, 2016(165) : 152-158
https://doi.org/10.1016/j.jinorgbio.2016.06.011
[6] Elastomeric free-form blood vessels for interconnecting organs on chip systems.
Lab Chip, 2016,16, 1579-1586
https://DOI.org/10.1039/C6LC00001K
[7] Controllable release of nitric oxide and doxorubicin from engineered nanospheres for synergistic tumor
therapy.
Acta Biomaterialia 2017, 57 : 498-510
https://doi.org/10.1016/j.actbio.2017.05.019
[8] Visible light-triggered nitric oxide release from near-infrared fluorescent nanospheric vehicles.
Analyst, 2014, 139:3398-3406
[9] Photocontrolled nitric oxide release from two nitrosylruthenium isomer complexes and their potential
biomedical applications.
J. Biomed. Opt. 2015,20(1):015004
[10] Hyperbranched polyether hybrid nanospheres with CdSe quantum dots incorporated for selective detection
of nitric oxide.
Materials Letters, 2014, 123:104–106
[11] Nitric oxide-releasing polymeric furoxan conjugates
Polym. Chem., 2015(6):7737-7748
产品用途
针对动物
用于心肌、脑、肾、肝等缺血再灌注模型研究,以及中药及新研发药物对再灌注损伤保护的研究;
用于高血压动物模型研究以及抗高血压药物作用机制的研究;
用于糖尿病实验动物模型建立的辅助研究以及药物治疗作用机制探究的筛选;末期血管内皮功能紊乱(ROS及氧化应激)及药物作用机制研究;
用于氧化应激与疾病的关系研究以及抗氧化应激药物的机制研究和筛选;
用于小动物线粒体代谢研究,包括O2消耗,NO、H2S和HPS产生的研究;
针对植物
用于水稻、棉花、麦子、玉米等经济作物以及拟南芥等
模式植物盐应激、酸应激研究;
用于水稻、棉花、麦子、玉米等经济作物浸渍胁迫,生
物胁迫研究;
用于水稻、棉花、麦子、玉米等经济作物高温低温胁
迫,低氧胁迫研究;
用于模式植物和经济作物光合作用研究;
用于植物体内信号分子NO、H 2 S和CO的研究;
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